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blog:ngs-best-practice [2018/03/07 14:19]
thoelken created
blog:ngs-best-practice [2018/05/18 15:46] (current)
thoelken
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 I found this very useful link, of what tools to use, why each step is necessary and example usage. I found this very useful link, of what tools to use, why each step is necessary and example usage.
 [[https://​training.vi-seem.eu/​images/​trainingMaterial/​LifeSciences/​NGSbestPracticeForCyI.pdf|vi-ssem.eu/​BestPractice.pdf]] [[https://​training.vi-seem.eu/​images/​trainingMaterial/​LifeSciences/​NGSbestPracticeForCyI.pdf|vi-ssem.eu/​BestPractice.pdf]]
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 +And some Gotchas:
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 +- [[https://​achri.blogspot.de/​2016/​02/​how-not-to-use-deseq2-for-illumina.html|Illumina stranded protocol reads can be from the antisense strand in some cases (I guess)]]
 +- [[http://​journals.plos.org/​plosone/​article?​id=10.1371/​journal.pone.0173023|Article:​ The impact of RNA secondary structure on read start locations on the Illumina sequencing platform]]
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